SYVN1 Rabbit Polyclonal Antibody for WB, ELISA - Q86TM6

Core Product Specifications and Parameters

Biological Background: SYVN1 Function and Localization

• SYVN1 (synoviolin, HRD1) is a RING-type E3 ubiquitin ligase that accepts ubiquitin from the ER-associated UBC7 E2 conjugating enzyme and transfers it to substrate proteins, targeting them for proteasomal degradation. Related references: PMID:12459480, PMID:12646171, PMID:12975321
• It is a central component of the endoplasmic reticulum-associated degradation (ERAD) pathway, which eliminates misfolded proteins from the ER to maintain cellular proteostasis. Related references: PMID:12459480, PMID:12646171, PMID:12975321
• SYVN1 protects cells from ER stress-induced apoptosis, underscoring its anti-apoptotic function.
• It promotes the degradation of normal short-lived proteins (e.g., SGK) and disease-associated proteins such as polyglutamine-expanded huntingtin (HTT) and unfolded GPR37, thereby providing neuroprotection. Related references: PMID:17141218
• SYVN1 sequesters the tumor suppressor p53/TP53 in the cytoplasm and promotes its ubiquitination and degradation, thereby negatively regulating p53-dependent transcription, cell cycle arrest, and apoptosis. Related references: PMID:17170702
• The protein localizes to the endoplasmic reticulum membrane and is widely expressed, with particularly high levels in liver and kidney; its expression is elevated in synovial tissues from rheumatoid arthritis patients.
• SYVN1 is a multi-pass transmembrane protein with zinc-finger domains and is subjected to phosphorylation, reflecting its involvement in stress signaling and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• For Western blot, use positive control lysates from human or mouse cell lines with confirmed SYVN1 expression, such as HeLa, HEK293, or liver/kidney tissue extracts.
• The predicted molecular weight is approximately 68 kDa (calculated 67.7 kDa from UniProt); include a pre-stained protein ladder and run a 10% SDS-PAGE gel.
• For ELISA applications, the immunogen (aa 500–600) targets the C-terminal region of SYVN1; consider using this peptide as a positive control.
• Due to the polyclonal nature of the antibody, validate each new lot for consistent performance in your specific assay.
• Cross-reactivity with mouse enables translational studies; however, confirm reactivity in your mouse model of interest.
• Avoid repeated freeze-thaw cycles; aliquot and store at -20°C or as recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, including this SYVN1 rabbit polyclonal antibody, to support research in ubiquitin-proteasome pathways, ER stress, and apoptosis. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, or bulk ancillary reagents, CamelBio streamlines your sourcing from concept to clinic. Our team also provides technical consulting to accelerate your diagnostic development.