Rabbit anti-UBC Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P0CG48

Core Product Specifications and Parameters

Biological Background: UBC (Polyubiquitin-C) Function and Localization

• UBC (Polyubiquitin-C) is a precursor protein that yields multiple ubiquitin units upon proteolytic processing, essential for maintaining cellular ubiquitin pools.
• Ubiquitin can exist in free (unanchored) forms or covalently attached to target proteins via isopeptide bonds, forming monoubiquitin or various polyubiquitin chains.
• The type of polyubiquitin chain linkage (e.g., Lys-48, Lys-63, Lys-11) determines its biological outcome, including proteasomal degradation, DNA repair, endocytosis, and NF-kappa-B signaling.
• Ubiquitin localizes to the cytoplasm, nucleus, and mitochondrion outer membrane, reflecting its diverse cellular roles.
• During ubiquitination, the acceptor ubiquitin’s C-terminus is positioned in the active site via direct interaction with E2 enzymes such as UBE2R2. Related references: PMID:38326650
• As a monoubiquitin, its C-terminal glycine can be recognized as a C-degron by CRL2 E3 ubiquitin-protein ligase complexes. Related references: PMID:39548056
• Key post-translational modifications include ADP-ribosylation and phosphorylation, and the protein features isopeptide bonds and a Ubl conjugation domain.

Experimental Guidance and Technical Tips

• The immunogen matches the N-terminal region of human UBC, potentially detecting both precursor and processed ubiquitin forms; validate reactivity in your specific sample system.
• For WB, the full-length precursor appears at ~77 kDa, but ubiquitin conjugates may produce higher molecular weight species or smears; include appropriate positive and negative controls.
• For IHC-P and IF/ICC, optimize antigen retrieval and fixation conditions; confirm target expression using published tissue or cellular localization data.
• In ELISA, test the antibody as a capture or detection reagent after careful pairing and optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a seamless pipeline of IVD raw materials, technical services, and consulting to support diagnostic manufacturers from concept to clinic. This anti-UBC rabbit polyclonal antibody is suited for ubiquitin pathway research across human, mouse, and rat samples. We offer validated antibody pairs, optimized polyclonal/monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your assay development.