Rabbit anti-Human/Mouse CD276/B7-H3 Monoclonal Antibody for Flow Cytometry - Q5ZPR3-2

Core Product Specifications and Parameters

Biological Background: CD276/B7-H3 Function and Localization

• CD276 (B7-H3) is encoded by the CD276 gene (also referred to as B7H3) on chromosome 15.
• Isoform 2 (UniProt Q5ZPR3-2) is a 4Ig-B7-H3 variant containing four immunoglobulin-like domains.
• The calculated molecular weight of isoform 2 is 33,791 Da, as per UniProt record.
• Alternative names for this protein include 4Ig-B7-H3, B7-H3, B7H3, B7RP-2, and CD276 antigen.

Experimental Guidance and Technical Tips

• The antibody is supplied as an conjugate and is intended for flow cytometry; for best results, titrate the antibody and include appropriate isotype controls.
• The immunogen corresponds to the extracellular domain (aa 29–245), which is expected to be accessible on live cells; consider live-cell staining protocols to preserve surface epitopes.
• Cross-reactivity with both human and mouse CD276 is reported; validate specificity in your experimental model using knockout controls or blocking peptides if available.
• Multiple protein weight bands (33 kDa, 52 kDa, 57 kDa) may reflect differential glycosylation or isoform expression; if adapting this antibody for western blot, optimize sample preparation and blocking conditions.
• This antibody has not been validated for other applications; users should thoroughly pilot any non-FC use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier IVD raw-material partner, providing diagnostic developers and researchers with a reliable one-stop sourcing solution. This anti-CD276/B7-H3 antibody supports immune-oncology research and assay development, and CamelBio can further supply validated antibody pairs, bulk ancillary reagents, and custom recombinant proteins to accelerate your project from concept to clinic.