Rabbit anti-Human CPT1A Monoclonal Antibody for Flow Cytometry (Intracellular) - P50416

Core Product Specifications and Parameters

Biological Background: CPT1A Function and Localization

• CPT1A, also known as carnitine O-palmitoyltransferase 1 liver isoform (CPT I, CPTI-L), is an 88 kDa mitochondrial outer membrane enzyme encoded by the CPT1A gene (GeneID 1374) (UniProt P50416).
• It catalyzes the transfer of long-chain fatty acyl-CoA onto carnitine, a rate-limiting step for mitochondrial fatty acid β-oxidation (PubMed:11350182, PubMed:14517221, PubMed:16651524). Related references: PMID:11350182 PMID:14517221 PMID:16651524
• Beyond its canonical acyltransferase activity, CPT1A possesses lysine succinyltransferase activity, modifying proteins such as ENO1 to regulate metabolism independently (PubMed:29425493). Related references: PMID:29425493
• CPT1A sustains the type I interferon response by recruiting ZDHHC4 to palmitoylate MAVS at mitochondria, promoting MAVS stabilization and antiviral signaling (PubMed:38016475). Related references: PMID:38016475
• Strongly expressed in kidney and heart, with lower levels in liver and skeletal muscle, consistent with its role in energy metabolism.
• Subcellularly localized to the mitochondrial outer membrane; contains transmembrane helices.
• Plays a role in hepatic triglyceride metabolism, inducible regulatory T-cell (iTreg) differentiation, and liver injury by promoting ROS-induced oxidative stress and modulating NLRP3 signaling.
• Alternative splicing yields multiple isoforms; post-translational modifications include acetylation, phosphorylation, and nitration.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 520-720 of human CPT1A, covering the C-terminal catalytic domain; consider this region when designing detection or competition assays.
• Validated for intracellular flow cytometry; use appropriate cell permeabilization buffers to access the mitochondrially localized target.
• For other applications (e.g., Western blot, immunofluorescence), validate in your sample system and optimize fixation/permeabilization steps as needed.
• Cross-reactivity confirmed for human; if targeting other species, check sequence homology and test experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our CPT1A antibody portfolio supports research in fatty acid metabolism and mitochondrial biology, with flexible supply options to meet your diagnostic development needs.