Rabbit anti-Human CD335/NKp46 Monoclonal Antibody for IF/ICC and FC - O76036

Core Product Specifications and Parameters

Biological Background: Natural cytotoxicity triggering receptor 1 (CD335/NKp46) Function and Localization

• The protein is a natural cytotoxicity triggering receptor (NCR1) also known as CD335, LY94, or NKp46. It belongs to the immunoglobulin superfamily and exists in multiple isoforms due to alternative splicing.
• It functions as a cytotoxicity-activating receptor that enhances the ability of activated natural killer (NK) cells to lyse tumor cells.
• CD335/NKp46 is selectively expressed on both resting and activated NK cells, serving as a specific marker for NK cell identification.
• The receptor is a type I transmembrane glycoprotein located on the cell membrane, with an extracellular region containing two immunoglobulin-like domains.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, contributing to its structural stability.
• The gene NCR1 maps to human chromosome 19 and encodes a protein with a predicted molecular weight of approximately 34.5 kDa, though observed bands may vary due to glycosylation (21-34 kDa range).

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (aa 22-254) of human CD335/NKp46, which is exposed on the cell surface; this design is suitable for detecting the native receptor.
• For immunofluorescence (IF/ICC), consider using acetone-fixed or paraformaldehyde-fixed cell preparations, and include a negative control lacking the primary antibody to assess background.
• For flow cytometry (FC), direct conjugation with eliminates the need for a secondary antibody; ensure the fluorophore is compatible with your instrument’s laser and filter configurations (excitation/emission similar to Alexa Fluor 594).
• Expressing cells or NK cell lines should be used as positive controls; freshly isolated human PBMCs can serve as a source of endogenous CD335/NKp46-expressing NK cells.
• Due to glycosylation heterogeneity, multiple bands may appear in western blotting; if WB is attempted, note that the epitope may be denatured by reducing and boiling conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated access to IVD raw materials, supporting diagnostic developers from discovery to commercialization. For NK cell research and immunophenotyping, we supply a range of validated monoclonal antibodies, matched antibody pairs, and ancillary reagents. Whether you need bulk antibodies, custom conjugation, or rare target sourcing, CamelBio is your reliable partner.