PE Rabbit anti-Mouse T-bet/Tbx21 Monoclonal Antibody for FC (intra) - Q9JKD8

Core Product Specifications and Parameters

Biological Background: T-bet/Tbx21 Function and Localization

• T-bet is a T-box transcription factor (TBX21) that serves as a master regulator of Th1 lineage commitment, driving IFN-gamma production and CXCR3 expression. Related references: PMID:10761931, PMID:17923685, PMID:21095589
• It activates Th1 genes by recruiting chromatin remodeling complexes and the super elongation complex to establish a permissive chromatin state. Related references: PMID:27292648
• T-bet represses Th17 differentiation by blocking RUNX1-mediated RORC activation, and Th2 differentiation by suppressing GATA3 and NFATC2. Related references: PMID:21151104, PMID:15662016, PMID:21690296, PMID:23616576
• It represses aberrant type-I IFN responses in Th1 cells and regulates antiviral B-cell responses and IgG2a switching. Related references: PMID:28623086, PMID:27430722
• T-bet is a nuclear protein and is modified by phosphorylation and ubiquitination (isopeptide bond).
• It is expressed specifically in T cells, including regulatory T cells. Related references: PMID:10761931, PMID:11087660, PMID:28607488
• The protein has a molecular weight of approximately 58 kDa (predicted 57852 Da from UniProt).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 321-530) of mouse T-bet, suitable for generating antibodies that detect intracellular epitopes.
• For intracellular flow cytometry, standard fixation and permeabilization protocols are recommended; validate in the relevant mouse T-cell model system (e.g., Th1-polarized CD4+ T cells).
• Consider using appropriate isotype controls and Fc blocking to ensure specific detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic and research teams, offering one-stop access to validated antibodies and ancillary reagents. This monoclonal antibody targets mouse T-bet, a key transcription factor in T-cell biology, supporting immunology and infectious disease assay development. Explore our portfolio for matched antibody pairs, bulk reagents, and custom sourcing solutions to accelerate your workflow.