PE Rabbit anti-Mouse Ly-49D Monoclonal Antibody for Flow Cytometry - Q60651

Core Product Specifications and Parameters

Biological Background: Ly-49D Function and Localization

• Ly-49D, also known as Killer cell lectin-like receptor 4 (Klra4), is a cell surface receptor primarily expressed on natural killer (NK) cells.
• It functions as an activating receptor that recognizes class I MHC molecules, playing a critical role in NK cell-mediated cytotoxicity and immune surveillance.
• The protein is a member of the Ly-49 family of C-type lectin-like receptors, which includes both activating and inhibitory receptors that finely tune NK cell responses.
• Ly-49D is a type II transmembrane protein with an extracellular C-type lectin domain and a cytoplasmic domain that associates with the immunoreceptor tyrosine-based activation motif (ITAM)-containing adaptor DAP12 for signal transduction.
• Alternative splicing generates multiple isoforms of Ly-49D, potentially modulating ligand binding and signaling.
• Post-translational modifications include glycosylation and disulfide bond formation, which are important for protein folding and ligand interaction.
• Keywords indicate involvement in cell adhesion, and it is anchored to the cell membrane via a signal-anchor mechanism.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 66-263), making this antibody suitable for detecting surface Ly-49D by flow cytometry.
• Consider titrating the antibody to determine the optimal concentration for your specific cell type (e.g., mouse splenocytes or purified NK cells).
• As Ly-49D expression can be modulated by activation state, include appropriate controls and validate in your experimental system.
• This PE-conjugated monoclonal antibody allows direct detection without a secondary antibody, minimizing background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to high-quality IVD raw materials like this anti-Ly-49D antibody. From optimized monoclonal antibodies to ancillary reagents, we support your NK cell research and immunoassay development needs. Our sourcing capabilities cover validated antibody pairs, bulk reagents, and rare target raw materials to accelerate your workflow.