PE Rabbit anti-Human CDKN2A/p16INK4a Monoclonal Antibody for FC (intra) - P42771 / Q8N726

Core Product Specifications and Parameters

Biological Background: CDKN2A/p16INK4a Function and Localization

• CDKN2A encodes the tumor suppressor p16INK4a, also known as cyclin-dependent kinase inhibitor 2A, which is a key regulator of cell cycle progression.
• p16INK4a functions by binding to CDK4 and CDK6, thereby inhibiting their interaction with D-type cyclins and preventing phosphorylation of the retinoblastoma protein (RB1), leading to cell cycle arrest at the G1/S checkpoint.
• Alternative splicing of CDKN2A produces multiple isoforms, including p16INK4a and p14ARF, which arise from distinct first exons and share only partial coding sequences; p14ARF acts through a separate tumor-suppressive pathway involving p53 stabilization.
• p16INK4a localizes to both the cytoplasm and nucleus, consistent with its role in shuttling between compartments to regulate CDK activity.
• Post-translational modifications include phosphorylation and acetylation, which may modulate protein stability and interactions with CDKs.
• Expression of p16INK4a is widespread in normal human tissues, but notably absent in brain and skeletal muscle; an isoform is specifically expressed in the pancreas.
• Mutations in CDKN2A are associated with familial melanoma and other cancer predisposition syndromes, including Li-Fraumeni syndrome.

Experimental Guidance and Technical Tips

• This PE conjugate is intended for intracellular flow cytometry; establish optimal cell permeabilization and fixation conditions to ensure epitope accessibility.
• The immunogen (aa 1-156) spans the full-length p16INK4a protein; verify specificity using cell lines with known CDKN2A expression or knockout controls.
• Include appropriate isotype controls and unstained cells to set gates accurately; consider co-staining with additional markers for cell cycle or senescence panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement. This PE anti-CDKN2A/p16INK4a antibody supports cell cycle and tumor suppression research, and CamelBio can further supply complementary reagents or custom conjugate formats to streamline your diagnostic development pipeline.