PE Rabbit anti-Human CD117/c-Kit Monoclonal Antibody for Flow Cytometry - P10721

Core Product Specifications and Parameters

Biological Background: CD117/c-Kit Function and Localization

• CD117, also known as c-Kit, SCFR, or PBT, is a transmembrane tyrosine-protein kinase receptor encoded by the KIT gene; alternative splicing produces multiple isoforms (UniProt: P10721).
• The primary ligand is stem cell factor (SCF/KITLG). Ligand binding induces receptor dimerization and autophosphorylation, activating downstream signaling cascades that regulate cell survival, proliferation, and differentiation in hematopoiesis, melanogenesis, gametogenesis, and mast cell development.
• Activated CD117 recruits and phosphorylates key adaptor proteins and enzymes including PIK3R1 (PI3K), GRB2, and PLCG1, leading to activation of AKT, RAS/MAPK, and STAT (STAT1, STAT3, STAT5) pathways, as well as production of second messengers DAG and IP3.
• CD117 is predominantly localized on the cell membrane, but can also be found in the cytoplasm upon internalization and trafficking.
• In testis, CD117 is detected in spermatogonia in the basal layer and in interstitial Leydig cells, and its expression is maintained in ejaculated spermatozoa (at protein level). Related references: PMID:20601678
• The protein is heavily glycosylated, contains extracellular immunoglobulin domains and an intracellular kinase domain, and is classified as a proto-oncogene; disulfide bonds and phosphorylation are key structural and functional features.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to PE, making it suitable for single-step flow cytometric detection of CD117 without the need for a secondary antibody.
• The immunogen corresponds to the extracellular region (aa 26–520) of human CD117, suggesting reactivity with the native surface receptor.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and instrument; include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.
• Validate expression in known CD117-positive human cell lines (e.g., TF-1, HMC-1) or primary hematopoietic cells.
• Since CD117 can be internalized upon ligand stimulation, consider the timing of fixation and staining when analyzing activated cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated supplier of IVD raw materials, providing one-stop access to high-quality antibodies, antigens, and ancillary reagents. For CD117/c-Kit related research in hematology and oncology, we offer PE-conjugated monoclonal antibodies, bulk formats, and tailored sourcing solutions to support diagnostic development from concept to commercialization. Contact us for validated antibody pairs and custom services.