[KO Validated] Anti-LDHA Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P00338

Core Product Specifications and Parameters

Biological Background: LDHA Function and Localization

• LDHA (L-lactate dehydrogenase A chain) is a key glycolytic enzyme that catalyzes the stereospecific interconversion of pyruvate and lactate, coupled with NADH/NAD+ turnover.
• Also known as LDH muscle subunit (LDH-M), it is predominantly expressed in anaerobic tissues such as skeletal muscle and liver, where high glycolytic flux is required.
• Localizes to the cytoplasm, consistent with its role in cytosolic metabolism.
• Undergoes multiple post-translational modifications including acetylation, phosphorylation, and ubiquitination, which may regulate its activity, stability, or interaction partners.
• Alternative splicing produces multiple isoforms, though their functional differences remain to be fully characterized.
• Mutations in LDHA are associated with glycogen storage disease XI (GSD11), highlighting its critical role in energy metabolism.
• The protein has a molecular weight of approximately 36.7 kDa (UniProt: 36689 Da), but the antibody detects a band between 26-39 kDa likely due to isoform variations or PTMs.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to amino acids 200-300 of human LDHA. This region is conserved across human, mouse, and rat, explaining the broad cross-reactivity.
• Recommended applications include Western blot (WB), immunofluorescence/ICC (IF/ICC), and ELISA. For WB, the detected band may appear between 26–39 kDa; considering the predicted MW of ~36.7 kDa, validate the band specificity in your sample system.
• Consider using appropriate tissue or cell lysates expressing LDHA, such as skeletal muscle or liver, for positive controls.
• For immunofluorescence, permeabilization is required as LDHA is cytoplasmic. Validate the staining pattern in relevant cell lines.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this LDHA mAb. Our comprehensive portfolio covers key metabolic enzymes and rare targets, supporting assay development from concept to clinic. For bulk reagent inquiries or custom sourcing of ancillary reagents, contact our team.