CD46 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P15529

Core Product Specifications and Parameters

Biological Background: CD46 Function and Localization

• CD46, also known as membrane cofactor protein (MCP), is a type I transmembrane glycoprotein and a member of the regulators of complement activation (RCA) gene cluster.
• Serves as a cofactor for complement factor I, mediating the cleavage of C3b and C4b to protect host cells from complement-mediated damage.
• Acts as a costimulatory factor for T-cells, inducing differentiation of CD4+ T-cells into regulatory T (Treg) type 1 cells that secrete IL-10 and suppress immune responses.
• Involved in fertilization by facilitating sperm-oocyte fusion; localizes to the inner acrosomal membrane of sperm.
• Functions as a receptor for multiple pathogens, including measles virus, adenovirus subgroup B2 and Ad3, human herpesvirus 6, and pathogenic bacteria such as Neisseria and Streptococcus pyogenes (PubMed:11260136, PubMed:11971006, PubMed:7708671). Also contributes to fiber protein-mediated infection by adenovirus types 55 and 14a (PubMed:32581096). Related references: PMID:11260136 PMID:11971006 PMID:7708671 PMID:32581096
• Ubiquitously expressed on all nucleated cells (absent from erythrocytes). Heavily glycosylated, contains multiple sushi domains and disulfide bonds; alternative splicing generates multiple isoforms.
• Associated with atypical hemolytic uremic syndrome (AHUS2) due to its critical complement regulatory role.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was generated against a recombinant protein corresponding to amino acids 40-320 of human CD46, encompassing the extracellular sushi domains critical for ligand and pathogen binding. It is suitable for Western blot, IHC-P, IF/ICC, and ELISA.
• The predicted molecular weight of CD46 is approximately 44 kDa, but due to heavy glycosylation, the observed band may appear at a higher molecular weight (50–70 kDa) by Western blot. Validate the actual band size in your sample system.
• CD46 is broadly expressed in human, mouse, and rat tissues (except erythrocytes). For IHC and IF, appropriate positive controls include kidney, spleen, or placental tissues.
• Consider using this antibody to probe CD46 expression in studies of complement regulation, T-cell costimulation, or host-pathogen interactions. Verify cross-reactivity in non-human species by sequence alignment of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this CD46 pAb, and bulk ancillary reagents to support diagnostic assay development. With a focus on complement regulation and infectious disease research, CamelBio can assist in sourcing rare target raw materials and provide technical consultation from concept to clinic.