Anti-USP15 Rabbit Polyclonal Antibody for WB, IP, IF/ICC, ELISA - Q9Y4E8

Core Product Specifications and Parameters

Biological Background: USP15 Function and Localization

• USP15 (Unph-2, Unph4) is a deubiquitinating enzyme belonging to the ubiquitin-specific protease family.
• It functions as a hydrolase that removes conjugated ubiquitin from target proteins, regulating pathways such as TGF-beta receptor signaling, NF-kappa-B, and the RNF41/PRKN pathway. Related references: PMID:16005295 PMID:17318178 PMID:19576224
• In TGF-beta signaling, USP15 deubiquitinates monoubiquitinated R-SMADs (SMAD1/2/3) to promote target gene activation, or alternatively stabilizes TGFBR1 via deubiquitination. Related references: PMID:21947082 PMID:22344298
• USP15 deubiquitinates histone H2B, potentially regulating gene expression and DNA repair. Related references: PMID:24526689
• It inhibits mitophagy by counteracting parkin, cleaving polyubiquitin chains on mitochondrial substrates such as MFN2. Related references: PMID:24852371
• USP15 is involved in endosome organization via deubiquitination of SQSTM1, and negatively regulates antifungal immunity by deubiquitinating CARD9. Related references: PMID:27368102 PMID:33093067
• USP15 localizes to the cytoplasm, nucleus, and mitochondria, and is expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary.

Experimental Guidance and Technical Tips

• Given the synthetic peptide immunogen, the antibody likely recognizes the specified epitope. Validate specificity in knockout or knockdown models where possible.
• For Western blotting, the USP15 protein runs at approximately 112 kDa. Use an appropriate gel percentage (e.g., 8% Tris-Glycine) and ensure thorough transfer for high molecular weight proteins. Consider a wet transfer at low voltage overnight.
• In immunoprecipitation, a synthetic peptide-derived pAb may perform well. Cross-link the antibody to Protein A/G beads if co-eluting antibody chains interfere with detection of target at ~112 kDa.
• For immunofluorescence/ICC, account for the dual nuclear and cytoplasmic/mitochondrial localization of USP15. Optimize permeabilization (e.g., 0.1% Triton X-100) to allow antibody access to all compartments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, streamlining supply from concept to clinic. This anti-USP15 antibody supports investigations into ubiquitin-dependent signaling and deubiquitinase-related pathways, with applications in cancer, immune regulation, and neurodegenerative disease research. For rare target raw materials or comprehensive technical consulting, we serve as your dedicated sourcing partner.