Anti-TAF1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P21675

Core Product Specifications and Parameters

Biological Background: TAF1 Function and Localization

• TAF1 is the largest subunit and core scaffold of the TFIID complex, crucial for RNA polymerase II transcription initiation. It nucleates complex assembly and forms a promoter DNA binding subcomplex with TAF2 and TAF7. Related references: PMID:33795473 PMID:25412659 PMID:27007846

• TAF1 functions as a Ser/Thr kinase, capable of autophosphorylation and transphosphorylation of transcription factors. It specifically phosphorylates TP53 on Thr-55, promoting MDM2-mediated degradation, and also targets GTF2A1 and GTF2F1. Related references: PMID:25412659 PMID:8625415

• Exhibits histone acetyltransferase activity, acetylating histones H3 and H4, thereby modulating chromatin structure and transcriptional activity. Related references: PMID:15870300

• Essential for cell cycle progression; loss of TAF1 function arrests cells in the G1 phase. Related references: PMID:11278496 PMID:15053879 PMID:2038334

• Contains a bromodomain and possesses intrinsic DNA-binding activity, contributing to its regulatory roles. Related references: PMID:25412659

• Primarily localized to the nucleus, consistent with its function in transcriptional regulation.

• Alternative initiation and alternative splicing generate multiple protein isoforms. Post-translational modifications include acetylation, phosphorylation, and ubiquitination.

• Mutations in TAF1 are associated with diseases such as dystonia, intellectual disability, and Parkinsonism, highlighting its critical role in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 990-1250) resides in a region predicted to be accessible. For Western blot, consider using nuclear extracts and verify a specific band around 213 kDa.
• For immunohistochemistry (IHC-P), antigen retrieval optimization and inclusion of appropriate positive (e.g., tonsil, testis) and negative controls are recommended to confirm specificity.
• Cross-reactivity with mouse and rat suggests utility in rodent model studies; validate in the relevant experimental system.
• As a polyclonal antibody, lot-to-lot consistency should be monitored, and titration may be required for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents. For transcriptional regulation and TAF1-related research, our supply chain supports procurement of rare targets and customized antibody production. From concept to clinic, CamelBio bridges your assay development needs with reliable material sourcing.