Anti-QKI Polyclonal Antibody for WB, IF/ICC, ELISA - Q96PU8

Core Product Specifications and Parameters

Biological Background: QKI Function and Localization

• QKI (Protein quaking; Hqk; HqkI) is a KH domain-containing RNA-binding protein encoded by the QKI gene. Multiple isoforms arise through alternative splicing, including nuclear and cytosolic forms with distinct regulatory roles.
• Acts as an RNA reader that recognizes and binds specific RNA sequences and modifications, regulating pre-mRNA splicing, circular RNA (circRNA) formation, mRNA export, stability, and translation. Related references: PMID:22398723 PMID:23630077 PMID:25768908
• Plays a central role in myelinization by protecting and stabilizing target mRNAs (e.g., MBP, SIRT2, CDKN1B), regulating nuclear export of MBP mRNA, and controlling MAG pre-mRNA alternative splicing during oligodendrocyte differentiation. Related references: PMID:16641098
• Cytosolic isoform QKI6 interacts with G3BP1 to promote localization of N7-methylguanine (m7G)-modified mRNAs into stress granules, suppressing translation under stress conditions. Related references: PMID:37379838
• Localizes to the nucleus, cytoplasm, cytosol, and stress granules depending on the isoform. Nuclear isoforms regulate splicing and transcription coactivation, while cytosolic isoforms mediate mRNA translation and stress granule recruitment.
• Post-translationally modified by phosphorylation, methylation, and ubiquitin conjugation, which may modulate its RNA-binding activity and subcellular distribution.
• Predominantly expressed in the frontal cortex of the brain; down-regulated in the brain of schizophrenic patients, implicating it in neurological disorders.

Experimental Guidance and Technical Tips

• The immunogen (recombinant fragment, amino acids 1–341 of human QKI, NP_006766.1) covers a region conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blotting (WB), expect a band near 38 kDa; consider using whole-cell lysates from brain or neural cell lines as positive controls.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize permeabilization and fixation to detect nuclear and/or cytosolic QKI pools, and validate specificity using knockout or knockdown models.
• For ELISA, pair with appropriate recombinant QKI protein standards and confirm performance in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, serving diagnostic manufacturers and research institutes from concept to clinic. This QKI Rabbit pAb supports investigations into RNA processing, myelinization, and neurological disease mechanisms. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your development pipeline.