Anti-PTRH2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y3E5

Core Product Specifications and Parameters

Biological Background: PTRH2 Function and Localization

• The PTRH2 protein, also known as BIT1 or PTH2, acts as a peptidyl-tRNA hydrolase, releasing tRNAs from the ribosome during protein synthesis and ensuring translation fidelity. Related references: PMID:14660562
• It promotes caspase-independent apoptosis by regulating the activity of transcriptional corepressors AES and TLE1.
• PTRH2 localizes to the mitochondrial outer membrane, linking mitochondrial function to apoptotic signaling.
• The protein contains a transmembrane helix, enabling its anchorage to the mitochondrial membrane.
• Subject to post-translational modifications, including ubiquitin-like conjugation (Ubl conjugation) as annotated in UniProtKB.
• Associated with the disease IMNEPD (infantile multisystem neurologic-endocrine-pancreatic disease), highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 40–179) excludes the N-terminal region; verify that the antibody recognizes endogenous full-length PTRH2 in your sample system.
• For Western blotting, a band near 19 kDa is expected; optimize blocking and antibody dilutions for best signal-to-noise.
• In IHC-P applications, consider heat-induced antigen retrieval and test primary antibody concentrations on control tissues.
• For IF/ICC, cellular permeabilization is required to detect mitochondrial outer membrane PTRH2.
• For ELISA formats, use standard coating buffers and validate cross-reactivity with relevant species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-PTRH2 polyclonal antibody, to support apoptosis and mitochondrial research. Beyond antibodies, we supply antigen raw materials, antibody pairs, and ancillary reagents, helping diagnostic manufacturers and labs streamline procurement from concept to clinic.