Anti-PKA RIIα (PRKAR2A) Rabbit Monoclonal Antibody for WB, ELISA - P13861

Core Product Specifications and Parameters

Biological Background: PRKAR2A Function and Localization

• PRKAR2A encodes the cAMP-dependent protein kinase type II-alpha regulatory subunit, also known as PKR2, which is a key component of the PKA holoenzyme complex.
• The protein contains two cAMP-binding domains and acts as the regulatory subunit that inhibits the catalytic activity of PKA in the absence of cAMP; upon cAMP binding, it dissociates and activates the kinase.
• Type II regulatory subunits mediate membrane association by binding to A-kinase anchoring proteins (AKAPs), thereby localizing PKA activity to specific subcellular compartments and integrating cAMP signaling with other pathways.
• PRKAR2A is one of four regulatory subunit isoforms (I-alpha, I-beta, II-alpha, and II-beta) whose expression varies across tissues, being either constitutive or inducible depending on cell type.
• Subcellularly, PRKAR2A is found in the cytoplasm and associates with the cell membrane, reflecting its role in compartmentalized cAMP signaling.
• The protein undergoes post-translational modifications including phosphorylation and acetylation, which may modulate its activity and interactions (UniProt keywords: Acetylation, Phosphoprotein).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 312-404) of human PRKAR2A, which includes part of the second cAMP-binding domain; consider this when evaluating isoform cross-reactivity.
• For Western blotting, detect a band at approximately 46 kDa in human cell lysates; validate the antibody in the relevant sample system to confirm specificity.
• This antibody is suitable for ELISA; optimal working dilutions should be determined experimentally by the end user.
• Because the antibody was raised against a recombinant fragment, it may recognize linear epitopes; for native protein detection, consider testing under conditions that preserve conformational structures.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-PRKAR2A clone. We streamline procurement of antibodies, antigen pairs, and ancillary reagents, and can assist in sourcing rare-target raw materials for cAMP signaling and kinase research applications. Our technical team is ready to help you move from concept to clinic with integrated supply and consulting services.