Anti-NFIX Rabbit Polyclonal Antibody for WB and ELISA - Q14938

Core Product Specifications and Parameters

Biological Background: NFIX Function and Localization

• Nuclear factor 1 X-type (NFIX) is a member of the nuclear factor I (NFI) family of transcription factors, encoded by the NFIX gene (GeneID: 4784).
• It functions as a CCAAT-box-binding transcription factor, recognizing the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' in viral and cellular promoters.
• NFIX is capable of activating both transcription and replication, notably binding to the origin of replication of adenovirus type 2.
• Subcellular localization is nuclear, consistent with its role as a transcription factor and DNA-binding protein.
• Post-translational modifications include phosphorylation, methylation, isopeptide bond formation, and ubiquitination, suggesting regulatory complexity.
• Associated with disease variants (e.g., Sotos syndrome 2) when mutated.
• Widely expressed across tissues, indicating broad regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 180-260) of NFIX, which is conserved between human and mouse, supporting the observed cross-reactivity. Validate reactivity in relevant cell lines.
• For Western blotting, probe for a band at ~55 kDa; consider using nuclear extract preparations from human (e.g., HeLa, HEK293) or mouse samples.
• For ELISA applications, the antibody may be used as a capture or detection reagent. Pairing with another specific antibody could enhance assay sensitivity.
• As a polyclonal antibody, lot-to-lot consistency should be verified, and positive/negative controls should be included in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For your NFIX research and assay development, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. From concept to clinic, CamelBio supports your transcription factor and DNA replication studies with reliable raw materials.