Anti-NEDD4L Rabbit Monoclonal Antibody for WB, ELISA - Q96PU5

Core Product Specifications and Parameters

Biological Background: NEDD4L Function and Localization

• NEDD4L is a HECT-type E3 ubiquitin-protein ligase that mediates polyubiquitination of target proteins, regulating processes such as autophagy, innate immunity, and DNA repair. Related references: PMID:20064473 PMID:31959741 PMID:33608556
• It inhibits TGF-beta signaling by triggering ubiquitination and proteasome-dependent degradation of SMAD2 and TGFBR1. Related references: PMID:15496141
• NEDD4L promotes ubiquitination and internalization of various plasma membrane channels, including ENaC, voltage-gated sodium channels (SCN2A, SCN3A, SCN5A, SCN9A, SCN10A), potassium channels (KCNA3, KCNH2, KCNQ2, KCNQ3), the glutamate transporter EAAT1, and the chloride channel CLC5. Related references: PMID:26363003 PMID:27445338
• It ubiquitinates and degrades SGK1, TNK2, ULK1, and ASCT2, impacting cell growth, autophagy, and TOR signaling. Related references: PMID:25631046 PMID:28820317 PMID:27694961
• In antiviral innate immunity, NEDD4L catalyzes Lys-29-linked cysteine ubiquitination of TRAF3, enhancing 'Lys-48' and 'Lys-63'-linked ubiquitination of TRAF3. Related references: PMID:33608556
• The NEDD4L protein belongs to the NEDD4 family of HECT-type E3 ubiquitin ligases and undergoes alternative splicing, producing multiple isoforms.
• NEDD4L localizes to the cytoplasm, Golgi apparatus, and endosome/multivesicular body compartments.
• It is ubiquitously expressed with highest levels in prostate, pancreas, and kidney, and is also present in melanocytes.

Experimental Guidance and Technical Tips

• The immunogen was designed from a synthetic peptide corresponding to amino acids 876-975 of human NEDD4L; this region may be conserved across isoforms, but confirm specificity in the relevant experimental system.
• For Western blot, NEDD4L migrates at approximately 112 kDa; ensure appropriate transfer conditions (e.g., extended transfer time or higher voltage) for detection of this high-molecular-weight protein.
• Validate NEDD4L expression in human cell lines or tissues with known high expression, such as kidney, prostate, or pancreas, to confirm antibody reactivity.
• When performing ELISA, include appropriate positive and negative controls to ensure specificity; consider testing a dilution series to optimize the working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers the NEDD4L rabbit monoclonal antibody as a reliable tool for researchers and diagnostic developers studying ubiquitin ligase pathways, ion channel regulation, and antiviral immunity. As your one-stop IVD raw-material sourcing partner, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—streamlining your journey from concept to clinic.