Anti-Mrpl46 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9EQI8

Core Product Specifications and Parameters

Biological Background: Mrpl46 Function and Localization

• Mrpl46 encodes the large ribosomal subunit protein mL46 (also known as 39S ribosomal protein L46, mitochondrial, or MRP-L46), an essential component of the mitochondrial ribosome.
• The protein is localized to the mitochondrion, consistent with its role in mitochondrial translation.
• Contains a transit peptide that directs it to the mitochondria for import.
• As a ribosomal protein, it contributes to the structural assembly and function of the mitochondrial large subunit (39S).
• Post-translational modifications include acetylation, which may regulate its function or interactions.
• A 3D-structure has been determined, providing insights into its structural role within the ribosome.
• The protein has a molecular weight of approximately 32 kDa (32,132 Da), matching the predicted size.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length mouse Mrpl46 (amino acids 1–283). As a polyclonal antibody raised in rabbit, it is expected to recognize multiple epitopes. For specificity validation, consider siRNA knockdown or knockout controls in your sample system.
• For Western blot, enrichment of mitochondrial fractions can improve the signal-to-noise ratio. The expected band is around 32 kDa.
• In IHC-P, mitochondrial detection may require optimized antigen retrieval protocols. Use tissues with high mitochondrial content as positive controls.
• For ELISA, the recombinant immunogen or synthetic peptides can serve as coating antigens. Titrate the antibody to achieve optimal sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials for diagnostic development. For mitochondrial research, our portfolio includes antibodies like anti-Mrpl46, enabling studies of mitochondrial translation and ribosome structure. From concept to clinic, we provide the technical services and reliable supply to accelerate your assay development and biomarker discovery.