Anti-MRPL15 Rabbit Polyclonal Antibody for WB, ELISA - Q9P015

Core Product Specifications and Parameters

Biological Background: MRPL15 Function and Localization

• MRPL15 (mitochondrial ribosomal protein L15), also designated L15mt or MRP-L15, is a component of the large 39S subunit of the mitochondrial ribosome.
• It is encoded by the MRPL15 gene in humans, with alternative names HSPC145, MRP-L7, and RPML7.
• The protein localizes exclusively to the mitochondrion, consistent with its role in mitochondrial protein synthesis.
• As a ribosomal protein of the uL15 family, it contributes to the structural integrity and function of the mitochondrial ribosome.
• The presence of a transit peptide annotation indicates that MRPL15 is synthesized as a precursor and imported into mitochondria.
• Proteomics identification supports its expression in human cells, and the protein has a calculated molecular weight of approximately 33 kDa.

Experimental Guidance and Technical Tips

• The antibody was raised against a peptide spanning residues 197-296 of human MRPL15 (NP_054894.1); verify that this region is conserved in your target species to ensure reactivity.
• For Western blotting, a band at ~33 kDa is expected; consider enriching mitochondrial fractions to improve detection.
• In ELISA applications, the immunizing peptide can serve as a positive control to confirm antibody specificity.
• Cross-reactivity with mouse and rat MRPL15 suggests conserved epitopes, but sequence alignment is advised for other model organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, supporting mitochondrial research and diagnostic development with validated reagents like this anti-MRPL15 polyclonal antibody. We offer comprehensive access to antibody pairs, optimized recombinant proteins, bulk ancillary reagents, and rare target sourcing to accelerate your projects from concept to clinic.