Anti-MAT1A Rabbit Polyclonal Antibody for WB, ELISA - Q00266

Core Product Specifications and Parameters

Biological Background: MAT1A Function and Localization

• MAT1A encodes S-adenosylmethionine synthase isoform type-1, also known as methionine adenosyltransferase 1 (MAT 1) or MAT I/III. It belongs to the AdoMet synthase family.
• The enzyme catalyzes the two-step synthesis of S-adenosylmethionine (AdoMet) from methionine and ATP: first formation of AdoMet and triphosphate, then hydrolysis of the triphosphate.
• AdoMet serves as the principal methyl donor in numerous biological methylation reactions, including DNA, RNA, and protein methylation, as well as polyamine synthesis and transsulfuration.
• MAT1A is primarily expressed in the liver, where it plays a critical role in hepatic one-carbon metabolism.
• The protein is a key enzyme of the one-carbon metabolic pathway and is associated with keywords such as ATP-binding, nucleotide-binding, magnesium and potassium metal-binding, and transferase activity.
• Structural studies and proteomics identification are available for this protein, which contains disulfide bonds and undergoes post-translational modifications including S-nitrosylation.
• Disease variants in MAT1A have been linked to liver-related disorders, highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 1–395 of human MAT1A, which covers the full-length protein. This design may enable recognition of native and denatured forms, suitable for both WB and ELISA applications.
• When performing Western blot with this polyclonal antibody, consider using denaturing conditions and validating the detected band at the expected molecular weight of ~44 kDa (predicted 43.6 kDa from sequence) in human, mouse, or rat liver lysates.
• For ELISA, the antibody can be used as a capture or detection reagent; optimize coating and detection conditions using recombinant MAT1A protein.
• Due to high sequence conservation, cross-reactivity with mouse and rat MAT1A is anticipated; however, individual validation in the relevant sample system is advisable.
• Given the liver-specific expression of MAT1A, liver tissue or hepatocyte-derived cell lines are recommended as positive controls.
• The antibody’s polyclonal nature may offer higher sensitivity but may require careful lot-to-lot consistency testing in critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to validated IVD raw materials like this MAT1A antibody, supporting studies in one-carbon metabolism and liver-related pathologies. Our comprehensive portfolio includes antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, ensuring a seamless transition from development to clinical applications.