Anti-macroH2A.1 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O75367

Core Product Specifications and Parameters

Biological Background: macroH2A.1 Function and Localization

• macroH2A.1 is a variant histone H2A that replaces conventional H2A in a subset of nucleosomes, where it represses transcription and contributes to nucleosome-mediated DNA compaction, limiting DNA accessibility to cellular machineries. Related references: PMID:12718888, PMID:15621527, PMID:16428466, PMID:15897469
• Involved in stable X chromosome inactivation and inhibits the binding of transcription factors, including NF-kappa-B, while interfering with SWI/SNF remodeling complexes. Related references: PMID:15897469, PMID:12718888, PMID:16428466
• Recruits class I HDACs and inhibits histone acetylation by EP300, inducing a hypoacetylated state of chromatin. Related references: PMID:16107708, PMID:16428466
• Isoform 1 specifically binds poly-ADP-ribose and O-acetyl-ADP-ribose, playing a key role in NAD+ metabolism by capping PARP1-generated ADP-ribose chains, thereby limiting NAD+ consumption and supporting mitochondrial respiration. It also increases expression of redox genes such as SOD3. Related references: PMID:15902274, PMID:23022728
• Isoform 2 does not bind poly-ADP-ribose and represses SOD3 gene expression. Related references: PMID:15902274, PMID:23022728
• Localized to the nucleus and chromosomes.
• Widely expressed across tissues.

Experimental Guidance and Technical Tips

• The antibody was generated against a peptide spanning amino acids 200–300, which includes the non-histone macro domain. Consider this region's conservation when assessing cross-reactivity with other macroH2A variants.
• For Western blotting, the predicted molecular weight is ~39 kDa. Due to the basic nature of histones, effective extraction may require specialized histone extraction protocols or acid-urea gels.
• Validate the antibody in the relevant sample systems (human, mouse, rat) for IHC-P, IF/ICC, or ELISA as appropriate, using appropriate controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We supply validated monoclonal antibodies like anti-macroH2A.1 to support chromatin and gene regulation research. Beyond individual antibodies, our portfolio includes bulk ancillary reagents, custom antibody development, and rare target sourcing to streamline your assay development workflow.