Anti-Lysozyme (LYZ) Monoclonal Antibody for WB, IHC-P, ELISA - P61626

Core Product Specifications and Parameters

Biological Background: Lysozyme C Function and Localization

• Lysozyme C (LYZ), also known as 1,4-beta-N-acetylmuramidase C, catalyzes the hydrolysis of peptidoglycan in bacterial cell walls, exerting bacteriolytic activity.
• The enzyme is primarily secreted and found in various tissues and body fluids, where it is associated with the monocyte-macrophage system and enhances the activity of immunoagents.
• Lysozyme C belongs to the glycoside hydrolase family 22, characterized by a conserved active site and stabilizing disulfide bonds; alternative names include LZM and LYZF1.
• It plays a key role in innate immunity and antimicrobial defense; UniProt keywords include Antimicrobial, Bacteriolytic enzyme, Secreted, Amyloid, and Disease variant.
• Mutations in the LYZ gene are linked to hereditary systemic amyloidosis, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human Lysozyme C (NP_000230.1); this N-terminal region is expected to be conserved across species, consistent with the observed cross-reactivity to mouse and rat.
• For Western blot (WB), the predicted molecular weight is approximately 17 kDa; consider using reducing sample buffer to ensure complete denaturation of disulfide-linked structures.
• In immunohistochemistry (IHC-P), validate staining patterns using known Lysozyme-positive tissues such as Paneth cells or macrophages as positive controls.
• For ELISA, pairing this antibody with appropriate capture/detection reagents is advised; performance should be verified in the relevant biological matrix.
• As experimental conditions can vary, it is recommended to optimize and validate the antibody in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, offering diagnostic manufacturers and research laboratories comprehensive access to validated antibodies, ancillary reagents, and custom procurement services. This anti-Lysozyme monoclonal antibody supports projects in infectious disease, immunology, and amyloidosis research. From monoclonal antibodies to bulk reagent supply, CamelBio bridges the gap from concept to clinical application.