Anti-ITGAX Polyclonal Antibody for WB, IF/ICC, ELISA - P20702

Core Product Specifications and Parameters

Biological Background: ITGAX Function and Localization

• Integrin alpha-X (ITGAX), also known as CD11C, is a member of the integrin alpha chain family, pairing with beta-2 to form the p150,95 leukocyte adhesion receptor.
• The alpha-X/beta-2 integrin serves as a receptor for fibrinogen, recognizing the G-P-R sequence, and mediates cell-cell interactions critical for inflammatory responses.
• ITGAX plays a key role in monocyte adhesion and chemotaxis, facilitating immune cell trafficking.
• It acts as a receptor for ICAM4 on erythrocytes, promoting erythrophagocytosis. Related references: PMID:16985175
• ITGAX is predominantly expressed on the surface of monocytes and granulocytes, consistent with its immune function.
• The protein localizes to the cell membrane and contains calcium/magnesium binding domains, disulfide bonds, and N-linked glycosylation sites, contributing to its structure and ligand binding.
• The predicted molecular weight is approximately 128 kDa (UniProt: 127.8 kDa).

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 146-342) lies within the extracellular domain, which is expected to generate antibodies recognizing the native protein.
• For Western blot, a band around 128 kDa should be detected in samples expressing ITGAX, such as THP-1 cells or primary monocytes. Validate the observed band using a relevant knockdown or blocking peptide.
• In immunofluorescence/immunocytochemistry, membrane staining is anticipated. Consider using monocyte/macrophage cell lines or isolated peripheral blood mononuclear cells.
• Cross-reactivity with mouse and rat has been reported, but conservation of the immunogen sequence across species should be verified before use.
• As a polyclonal antibody, lot-to-lot variability may occur. It is advisable to set up and optimize the assay with a pilot quantity before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials. For ITGAX (CD11C)-related projects in inflammation and immune cell adhesion, we provide validated polyclonal and monoclonal antibodies, matched antibody pairs, and ancillary reagents. Whether you need bulk quantities of this specific antibody, custom antibody development for rare targets, or integrated technical consulting, CamelBio streamlines your path from concept to clinical application.