Anti-IRF3 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q14653

Core Product Specifications and Parameters

Biological Background: IRF3 Function and Localization

• IRF3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon-dependent immune responses, critical for innate immunity against DNA and RNA viruses. Related references: PMID:22394562 PMID:24049179 PMID:25636800
• It controls transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISGs) by binding to the ISRE promoter element. Related references: PMID:11846977 PMID:16846591 PMID:16979567
• IRF3 is a more potent activator of IFNB than IFNA and functions in both early and late phases of IFN gene induction. Related references: PMID:16846591 PMID:16979567 PMID:20049431
• In unstimulated cells, IRF3 is inactive in the cytoplasm; viral infection or TLR signaling induces its phosphorylation by IKBKE and TBK1, leading to dimerization, nuclear translocation, and formation of the DRAF1 transcription complex with CREBBP. Related references: PMID:22394562 PMID:25636800 PMID:27302953 PMID:16154084 PMID:33440148
• IRF3 can activate distinct gene expression programs in macrophages and induce apoptosis in primary macrophages; during Sendai virus infection, it is recruited to mitochondria via TOMM70:HSP90AA1, forming an apoptosis complex with BAX. Related references: PMID:16846591 PMID:25609812
• It serves as a key transcription factor regulating the IFN response during SARS-CoV-2 infection. Related references: PMID:33440148
• IRF3 undergoes post-translational modifications such as phosphorylation, acetylation, and ubiquitination, and contains disulfide and isopeptide bonds.
• It localizes to the cytoplasm, nucleus, and mitochondrion, and is constitutively expressed in a variety of tissues.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is ~47 kDa; validate expression in relevant cell lines or tissues known to express IRF3, such as immune cells.
• In immunofluorescence assays, consider using positive controls that stimulate IRF3 nuclear translocation (e.g., poly(I:C) treatment or viral infection) to confirm antibody specificity.
• This antibody may be suitable for immunoprecipitation; optimize lysis buffer conditions to maintain protein interactions and confirm detection with appropriate secondary antibodies.
• Cross-reactivity with mouse and rat enables comparative studies, but validate in your specific experimental model as sequence homology may affect binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive range of IVD raw materials, technical services, and developmental consulting. For innate immunity research, this IRF3 polyclonal antibody complements our portfolio of optimized monoclonal and polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents. Contact us to explore custom sourcing solutions and accelerate your diagnostic assay development.