Anti-H6PD Rabbit Polyclonal Antibody for WB, ELISA - O95479

Core Product Specifications and Parameters

Biological Background: H6PD Function and Localization

• The H6PD gene encodes the GDH/6PGL endoplasmic bifunctional protein, also known as hexose-6-phosphate dehydrogenase.
• It catalyzes the first two steps of the oxidative branch of the pentose phosphate pathway within the ER lumen, converting glucose-6-phosphate to 6-phosphogluconate with NADPH production. Related references: PMID:12858176, PMID:18628520, PMID:23132696
• Exhibits broader substrate specificity than G6PD, acting on glucosamine 6-phosphate and glucose 6-sulfate as alternative substrates.
• The NADPH generated is essential for maintaining reductive cofactor levels in the oxidizing ER environment and indirectly regulates enzymes like HSD11B1 (corticosteroid 11-beta-dehydrogenase isozyme 1). Related references: PMID:18628520
• Subcellularly localized to the endoplasmic reticulum lumen, consistent with its signal peptide and role in ER redox homeostasis.
• Highly expressed in liver, with widespread tissue distribution, reflecting its metabolic importance.
• Post-translational modifications include glycation and pyroglutamate formation, as indicated by its glycoprotein status and pyrrolidone carboxylic acid keyword.
• Genetic variants in H6PD are associated with cortisone reductase deficiency (CORTRD1), underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 20–200 of human H6PD, which may detect both full-length and truncated forms; perform appropriate size validation in western blotting.
• For WB, the expected molecular weight is ~89 kDa, but consider potential glycosylation-induced shifts in migration; run appropriate controls.
• This polyclonal antibody is suitable for ELISA; due to polyclonal nature, it may offer high sensitivity but validate in your specific sandwich or direct assay format.
• Reported cross-reactivity with mouse and rat makes it useful for comparative studies, but always confirm reactivity in lysates from your specific tissue or cell line.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD raw materials, connecting diagnostic developers with validated antibodies, antigens, and ancillary reagents. The H6PD Rabbit pAb is part of our catalog supporting metabolic disorders and ER stress research. For bulk orders or custom antibody development, please contact our technical team.