Anti-GPX4 Rabbit Monoclonal Antibody (KD Validated) for WB, IF-P, IHC-P, ELISA - P36969

Core Product Specifications and Parameters

Biological Background: GPX4 Function and Localization

• GPX4, encoded by the GPX4 gene, is a member of the glutathione peroxidase family and is also known as phospholipid hydroperoxide glutathione peroxidase (PHGPx) and sperm nucleus glutathione peroxidase (snGPx).
• It functions as a vital antioxidant enzyme that directly reduces phospholipid hydroperoxides within lipid membranes, thereby preventing lipid peroxidation and protecting cells from oxidative damage (PubMed:40281343).
  Related references: PMID:40281343
• A critical role of GPX4 is the prevention of ferroptosis, a non-apoptotic, iron-dependent form of cell death resulting from the accumulation of lipid reactive oxygen species (PubMed:24439385, PubMed:40281343).
  Related references: PMID:24439385, PMID:40281343
• GPX4 is localized to both the mitochondria and the cytoplasm.
• The enzyme is primarily expressed in the testis and is also present in platelets, where it participates in arachidonic acid metabolism (PubMed:11115402).
  Related references: PMID:11115402
• GPX4 contains a selenocysteine residue at its active site, which is essential for its catalytic activity and resistance to overoxidation (By similarity).
• Beyond phospholipids, GPX4 can reduce fatty acid-derived hydroperoxides and small soluble peroxides such as H2O2 and cumene hydroperoxide (PubMed:11115402, PubMed:17630701, PubMed:36608588).
  Related references: PMID:11115402, PMID:17630701, PMID:36608588
• Additional reported roles include support of T-cell expansion during infection, sperm development, and photoreceptor survival (By similarity).

Experimental Guidance and Technical Tips

• As a KD-validated monoclonal antibody, this product is well-suited for Western blot applications where endogenous GPX4 detection can be confirmed using knockdown controls. Expect a band near 22 kDa.
• For immunofluorescence on paraffin-embedded (IF-P) or immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution conditions using positive control tissues such as testis or cell lines known to express GPX4.
• The antibody’s cross-reactivity with mouse and rat GPX4 makes it applicable for translational studies in these species; validate cross-reactivity in your specific model system.
• For ELISA, consider using this antibody as a capture or detection reagent in sandwich ELISA development for GPX4 quantification.
• Given its role in ferroptosis, this antibody can be employed to monitor GPX4 expression changes in response to ferroptosis inducers or inhibitors.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated monoclonal antibodies like this anti-GPX4 clone for applications in ferroptosis and oxidative stress research. Our one-stop supply chain covers antibody pairs, bulk ancillary reagents, and rare-target sourcing, accompanied by technical consulting to support your diagnostic development from concept to clinic. Pair this GPX4 antibody with our optimized reagent systems to streamline your assay workflows.