Anti-FTSJ1 Polyclonal Antibody for WB, ELISA - Q9UET6

Core Product Specifications and Parameters

Biological Background: FTSJ1 Function and Localization

• FTSJ1 encodes a tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase that specifically methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop, a modification critical for tRNA stability and accurate translation. Related references: PMID:25404562, PMID:26310293, PMID:32198346
• The methyltransferase activity requires two distinct cofactors: THADA for methylation at position 32 and WDR6 for methylation at position 34, highlighting a bipartite regulatory mechanism for tRNA modification. Related references: PMID:25404562, PMID:26310293, PMID:32558197, PMID:33771871
• FTSJ1 enhances translation efficiency of the UUU codon, particularly important for phenylalanine-rich proteins, and is requisite for overall cytoplasmic translational fidelity. Related references: PMID:32558197, PMID:32393790
• In neurogenesis, FTSJ1 is essential; it supports the expression of genes involved in neuronal development, mitochondrial translation and energy generation, and lipid biosynthesis, and it is necessary for RNA-mediated gene silencing. Related references: PMID:36720500, PMID:33771871
• FTSJ1 modifies a broad range of tRNA substrates, including tRNA(Phe), tRNA(Leu), tRNA(Trp), and others, at either position 32 or 34, impacting codon-anticodon interactions. Related references: PMID:26310293, PMID:32198346, PMID:32558197
• The protein localizes to both the cytoplasm and the nucleus, consistent with its roles in tRNA processing and possibly nuclear RNA regulation.
• It is widely expressed across tissues, with highest levels in adult heart and liver, and lower expression in brain, lung, skeletal muscle, kidney, and pancreas. In fetal tissues, it is detected in brain, lung, liver, and kidney, suggesting developmental importance.
• Mutations in FTSJ1 are linked to X-linked intellectual disability, underscoring its critical role in cognitive development and brain function.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein spanning amino acids 170-329 of human FTSJ1, a region within the catalytic methyltransferase domain. This immunogen design suggests the antibody likely recognizes epitopes critical for enzyme function. For confirming specificity, consider using the recombinant immunogen as a positive control in ELISA or as a blocking agent in western blot.
• This polyclonal antibody is validated for Western blot (WB) and ELISA. In WB, expect a band around 36 kDa (predicted molecular weight 36079 Da). Due to the polyclonal nature, multiple bands may appear; optimize blocking and antibody dilution for your specific sample type. For ELISA, the recombinant protein can serve as a standard.
• The manufacturer reports reactivity with human, mouse, and rat samples. When working with these species, it is advisable to validate the antibody in your particular experimental conditions, as cross-reactivity can vary depending on sample preparation and assay parameters.
• Upon arrival, the antibody is shipped on ice bags. Store at -20°C or according to the manufacturer's recommendations, and avoid repeated freeze-thaw cycles to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibodies like anti-FTSJ1, to support diagnostic development from concept to clinic. We provide one-stop access to optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for tRNA modification and neurogenesis research. Our supply chain capabilities ensure reliable delivery of high-quality raw materials for your assay development needs.