Anti-FGF23 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q9GZV9

Core Product Specifications and Parameters

Biological Background: Fibroblast growth factor 23 Function and Localization

• FGF23 is a critical endocrine regulator of phosphate homeostasis, controlling renal phosphate excretion. Related references: PMID:11062477
• It inhibits renal tubular phosphate transport by downregulating SLC34A1 expression. Related references: PMID:11409890
• The protein also modulates vitamin D metabolism, contributing to systemic mineral balance. Related references: PMID:15040831
• In the presence of α-Klotho, FGF23 up-regulates EGR1 and directly suppresses parathyroid hormone secretion from the parathyroid gland.
• FGF23 negatively regulates osteoblast differentiation and bone matrix mineralization, linking bone remodeling to phosphate handling. Related references: PMID:18282132
• It is predominantly expressed by osteocytes and osteogenic cells in bone and is secreted into the bloodstream.
• Post-translational modifications include glycosylation and phosphorylation, and it possesses a signal peptide and disulfide bonds essential for its structure.
• Mutations in FGF23 cause human diseases such as autosomal dominant hypophosphatemic rickets (ADHR) and hyperphosphatemic familial tumoral calcinosis (HFTC2), reflected in its alternate names.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 28 kDa; validate the observed band in your sample system and consider using appropriate positive controls.
• In immunofluorescence/immunocytochemistry (IF/ICC), human osteoblast or osteocyte cell lines may serve as suitable positive controls; optimize fixation and permeabilization conditions.
• For ELISA applications, pairing with a suitable detection antibody may be necessary; perform assay optimization according to your protocol.
• Since this monoclonal antibody was raised against a recombinant fragment covering the mature protein, it may detect both full-length and processed forms; confirm specificity in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to validated antibodies, including this anti-FGF23 monoclonal antibody, for studying phosphate regulation and bone metabolism. Our portfolio includes optimized monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents to support assay development. For rare or specialized targets, we offer custom sourcing and technical consultancy from concept to clinical application.