Anti-ERH Polyclonal Antibody for WB and ELISA - P84090

Core Product Specifications and Parameters

Biological Background: ERH Function and Localization

• ERH (Enhancer of rudimentary homolog), also known as DROER, is a conserved nuclear protein encoded by the ERH gene in humans.
• Its primary function is linked to cell cycle regulation, though detailed mechanisms remain to be fully elucidated.
• ERH is ubiquitously expressed across all tissues examined, suggesting a fundamental cellular role.
• The protein localizes predominantly to the nucleus, consistent with its potential involvement in nuclear processes.
• ERH undergoes several post-translational modifications including acetylation, phosphorylation, and attachment of ubiquitin-like molecules (Ubl conjugation) via isopeptide bonds, indicating dynamic regulation.
• It has been identified through direct protein sequencing and high-throughput proteomics, and its 3D structure has been solved, facilitating structural-functional studies.

Experimental Guidance and Technical Tips

• The immunogen consists of full-length human ERH (aa 1-104), so the antibody is expected to detect the native protein as well as isoforms or fragments containing this region. For Western blot, expect a band around 12 kDa; use appropriate positive controls such as human cell lines known to express ERH.
• Since this is a polyclonal antibody, performance may vary between lots; we recommend titrating the antibody in your specific assay system and including proper loading and negative controls.
• For ELISA, the antibody can be used as a capture or detection reagent under standard conditions; however, optimization of coating concentrations and blocking steps is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, providing validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. For projects involving cell cycle regulation or nuclear protein targets like ERH, we can support your procurement needs from discovery to clinical application. Contact us to discuss your specific raw-material requirements and benefit from our streamlined supply chain and technical expertise.