Anti-EPB41 Rabbit Polyclonal Antibody for WB, IP, ELISA - P11171

Core Product Specifications and Parameters

Biological Background: EPB41 Function and Localization

• Protein 4.1 (EPB41, 4.1R) is a multifunctional structural protein originally identified as a major component of the erythrocyte membrane skeleton.
• It stabilizes the spectrin–actin network, thereby regulating the mechanical stability and deformability of red blood cells.
• In non-erythroid cells, EPB41 is required for the cortical recruitment of the dynein-dynactin complex and NUMA1 during anaphase, ensuring proper mitotic progression (PubMed:23870127).
  Related references:
  PMID:23870127
• EPB41 interacts with calmodulin and contains binding sites for actin, spectrin, and other membrane-associated proteins, and undergoes extensive alternative splicing to generate multiple isoforms.
• Post-translational modifications, including phosphorylation and O-glycosylation, regulate its association with the cytoskeleton and membrane.
• Subcellular localization includes the cytoplasm, cell cortex, cytoskeleton, and nucleus, reflecting its diverse functional roles.
• Mutations in EPB41 are associated with hereditary elliptocytosis and pyropoikilocytosis, hemolytic anemias characterized by abnormally shaped and fragile erythrocytes.
• The protein has a molecular weight of approximately 97 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band around 97 kDa; validate expression in erythrocyte lysates or relevant nucleated cell lines.
• When performing immunoprecipitation, use appropriate lysis buffers that preserve cytoskeletal interactions; consider a low-speed centrifugation step to pre-clear the lysate.
• Since the immunogen is a peptide from the 700–800 amino acid region, specificity can be confirmed by siRNA-mediated knockdown or by comparing with a cell line lacking EPB41 expression.
• For ELISA, optimize coating conditions and consider using recombinant protein fragments as standards.
• This polyclonal antibody may recognize multiple EPB41 isoforms due to alternative splicing; verify the identity of detected bands by mass spectrometry or isoform-specific probes.

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