Anti-EEF2K Polyclonal Antibody for WB, IF/ICC, ELISA - O00418

Core Product Specifications and Parameters

Biological Background: EEF2K Function and Localization

• EEF2K is a threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. It phosphorylates eukaryotic elongation factor 2 (EEF2) at a single site, rendering EEF2 unable to bind ribosomes and thus inactivating it. This reduces the overall rate of protein synthesis.
• EEF2K, also known as calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, is activated by upstream kinases such as AMPK and TRPM7. This activation links cellular energy status and calcium signaling to translational control.
• The kinase belongs to the serine/threonine-protein kinase family and requires calcium/calmodulin for its activity. It plays a critical role in cellular stress responses by modulating translation elongation.
• EEF2K has an ATP-binding site and undergoes phosphorylation, acetylation, and other post-translational modifications, as indicated by keywords including "Phosphoprotein" and "Acetylation".
• The protein has a molecular weight of approximately 82kDa and is classified under the transferase group, specifically transferring phosphorus-containing groups (kinase activity).
• As indicated by the UniProt keyword "3D-structure", structural information is available for EEF2K, which aids in understanding its kinase mechanisms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region spanning amino acids 406-725 of human EEF2K. This region includes catalytic and regulatory domains, which may contribute to the antibody's ability to recognize endogenous EEF2K in denaturing (WB) and native (IF/ICC) conditions. However, validation in the specific experimental context is recommended.
• This antibody has been validated for knockout (KO) studies, as indicated by "[KO Validated]" in the product name, confirming its specificity for EEF2K. For applications like WB, use appropriate KO cell lysates as negative controls to confirm band specificity.
• For IF/ICC, optimize fixation and permeabilization conditions, as EEF2K localization may be influenced by cell type and activation state. Co-staining with calmodulin or ribosomal markers could aid in co-localization studies.
• For ELISA, consider using the recombinant protein fragment (aa 406-725) as a positive control and validate with biological samples, ensuring minimal cross-reactivity with other kinases.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. The Anti-EEF2K polyclonal antibody supports research into translation regulation and kinase signaling, fitting seamlessly into diagnostic and research workflows. Partner with CamelBio for comprehensive procurement support from concept to clinic.