Anti-CYB5B Polyclonal Antibody for WB, ELISA - O43169

Core Product Specifications and Parameters

Biological Background: CYB5B Function and Localization

• CYB5B, also known as OMB5 or CYB5-M, is a member of the cytochrome b5 family and encodes the outer mitochondrial membrane isoform.
• It functions as a membrane-bound hemoprotein that serves as an electron carrier for several membrane-bound oxygenases.
• The protein contains a heme-binding domain that coordinates an iron atom, essential for its electron transport activity.
• CYB5B is anchored to the mitochondrial outer membrane via a C-terminal transmembrane helix.
• Post-translational modifications include acetylation, methylation, and phosphorylation, which may regulate its function or localization.
• Keywords associated with CYB5B include electron transport, heme, iron, metal-binding, and transport, reflecting its role in cellular redox processes.
• The calculated molecular weight of the mature CYB5B protein is approximately 16.7 kDa.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein encompassing the N-terminal region (aa 16-150), which includes part of the heme-binding domain; thus, it may recognize both native and denatured forms under reducing conditions.
• For Western blot, expect a band around 17 kDa. Verify specificity using appropriate positive and negative controls (e.g., human or mouse tissue/cell lysates known to express CYB5B).
• For ELISA, consider coating with recombinant CYB5B or cell lysates; optimal dilution should be determined empirically.
• Cross-reactivity with human and mouse has been reported; reactivity with other species should be validated based on sequence homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with reliable access to premium IVD raw materials, including validated antibodies like this CYB5B polyclonal antibody for mitochondrial research. We offer one-stop sourcing for antibody pairs, bulk ancillary reagents, and rare target raw materials to accelerate your assay development from concept to clinic.