Anti-CDC42 Rabbit Monoclonal Antibody for WB and ELISA - P60953

Core Product Specifications and Parameters

Biological Background: CDC42 Function and Localization

• Cell division control protein 42 homolog (CDC42) is a small GTPase of the Rho family that cycles between active GTP-bound and inactive GDP-bound states, regulating epithelial cell polarization and mitotic spindle attachment to kinetochores. Related references: PMID:15642749
• CDC42 is crucial for cell migration and neuronal filopodia formation, modulating actin cytoskeleton dynamics in response to extracellular signals. Related references: PMID:14978216, PMID:17038317
• It is required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons, and facilitates podosome formation in podocytes upon DOCK11 activation. Related references: PMID:33523862
• CDC42 participates in phagocytosis by organizing the F-actin cytoskeleton to form phagocytic cups. Related references: PMID:26465210
• Upon activation by PLEKHG4B, CDC42 contributes to actin cytoskeletal remodeling at epithelial cell-cell junctions. Related references: PMID:33310911
• CDC42 negatively regulates polymerization of SEPTIN12, suggesting a role in spermatogenesis. Related references: PMID:30189608
• Subcellular localization includes the cell membrane, cytoplasm, cytoskeleton, centrosome, spindle, midbody, dendrites, and acrosome, reflecting its diverse functions.
• The protein is subject to multiple post-translational modifications, including phosphorylation, prenylation, methylation, and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 60–191 of human CDC42, a region highly conserved among mammals, which likely accounts for the observed cross-reactivity with mouse and rat.
• For Western blot, a band at approximately 21 kDa should be detected; consider using appropriate positive controls (e.g., HeLa or A549 lysates) and optimize primary antibody concentration empirically.
• In ELISA, include the recombinant protein as a standard and validate assay performance with known positive and negative samples under controlled conditions.

CamelBio: Your One-Stop Sourcing Bridge

As a global IVD raw material supplier, CamelBio supports diagnostic manufacturers and researchers with high-quality antibodies like this CDC42 monoclonal antibody for applications in cell biology and oncology research. We offer a full portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents, along with customized sourcing for rare and emerging targets to accelerate your assay development from concept to clinic.