Anti-CD355/CRTAM Monoclonal Antibody for Flow Cytometry - O95727

Core Product Specifications and Parameters

Biological Background: Cytotoxic and Regulatory T-cell Molecule Function and Localization

• CRTAM, also known as Class-I MHC-restricted T-cell-associated molecule, is a single-pass type I membrane protein belonging to the immunoglobulin superfamily. It exists in multiple isoforms due to alternative splicing.
• Mediates heterophilic cell-cell adhesion that regulates activation, differentiation, and tissue retention of various T-cell subsets. Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and IFN-gamma secretion by CD8+ T-cells in vitro, as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo. Related references: PMID:15811952
• Regulates CD8+ T-cell proliferation in response to TCR activation but appears dispensable for CD8+ T-cell-mediated cytotoxicity.
• Interaction with SCRIB promotes the late phase of cellular polarization in a subset of CD4+ T-cells, regulating TCR-mediated proliferation and cytokine production (IFNG, IL17, IL22).
• By interacting with CADM1 on CD8+ dendritic cells, regulates the retention of activated CD8+ T-cells within the draining lymph node.
• Required for intestinal retention of various T-cell subsets and promotes adhesion to gut-associated CD103+ dendritic cells, facilitating the expression of homing molecules and conversion of CD4+ T-cells into CD4+ CD8+ T-cells.
• Localized at the cell membrane.
• Tissue expression restricted to activated class-I MHC-restricted cells, including NKT and CD8 T-cells. Strongly expressed in spleen, thymus, small intestine, peripheral blood leukocytes, and Purkinje neurons in the cerebellum.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 18–287), making this antibody well-suited for detecting surface-expressed CRTAM by flow cytometry.
• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to ensure specific staining.
• Validate the antibody in a relevant human sample system, such as activated CD8+ T-cells or NKT cells, where CRTAM expression is expected.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a seamless, one-stop sourcing experience for IVD raw materials. This anti-CD355/CRTAM monoclonal antibody supports studies in T-cell and NK cell biology, and we can deliver stable supplies of validated antibody reagents, custom developments, and ancillary reagents to streamline your workflow from concept to clinic.