Anti-CD201 Rabbit Polyclonal Antibody for WB, ELISA - Q64695

Core Product Specifications and Parameters

Biological Background: CD201 Function and Localization

• CD201, also known as endothelial protein C receptor (EPCR), activated protein C receptor, centrocyclin, or centrosomal protein CCD41, is a transmembrane receptor encoded by the Procr gene in mouse.
• It functions as a receptor for activated protein C, playing a pivotal role in the protein C anticoagulant pathway by enhancing protein C activation via the thrombin-thrombomodulin complex, thereby regulating blood coagulation.
• CD201 is a member of the MHC class I-like receptor family and is characterized by a signal peptide, an extracellular domain containing two disulfide bonds, a single transmembrane helix, and a short cytoplasmic tail.
• Subcellularly, CD201 localizes to the plasma membrane, consistent with its role as a cell surface receptor, and is also reported in association with centrosomes, as reflected by the alias CCD41.
• Tissue expression is predominantly restricted to endothelial cells, highlighting its specialized function in vascular biology.
• Post-translational modifications include N-linked glycosylation and the presence of conserved disulfide bonds, which contribute to structural stability and ligand binding.
• Keywords such as blood coagulation, hemostasis, disulfide bond, glycoprotein, membrane, receptor, and transmembrane helix underscore its involvement in hemostatic balance and membrane-bound signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 18–210 of mouse CD201, encompassing the predicted extracellular domain, which may recognize both the native membrane-bound form and soluble shed receptor in Western blot and ELISA.
• For Western blot, expect a band near the calculated molecular weight of 27 kDa under reducing conditions; however, glycosylation may lead to slightly higher apparent masses in some sample types. Validate the observed band using appropriate mouse endothelial cell lines or tissue lysates.
• For ELISA, pairing with a suitable detection antibody recognizing a non-overlapping epitope is advised to ensure specificity; consider optimizing coating and blocking conditions for mouse-derived samples.
• The antibody is reported to react with mouse CD201; cross-reactivity with orthologs from other species should be verified experimentally if extending applications.
• Always include positive and negative controls, such as recombinant CD201 protein or EPCR-overexpressing cells, to confirm assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive one-stop platform for IVD raw-material procurement, spanning from early-stage development to clinical application. For research on coagulation, hemostasis, and endothelial biology centered on targets like CD201/EPCR, we provide a reliable supply of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets. By integrating technical consultation with efficient material supply, CamelBio streamlines your transition from concept to clinic, ensuring you have the critical resources needed to advance your assays.