Anti-BBS7 Rabbit Polyclonal Antibody for WB/IF/ICC/ELISA - Q8IWZ6

Core Product Specifications and Parameters

Biological Background: BBS7 Function and Localization

• BBS7 is a core component of the BBSome, a multi-protein complex essential for ciliary membrane biogenesis and protein trafficking.
• It is expressed as two isoforms via alternative splicing; isoform 1 is detected in retina, lung, liver, testis, ovary, prostate, small intestine, brain, heart, and pancreas, while isoform 2 is ubiquitously expressed.
• The BBSome acts as a coat complex that recognizes sorting signals on membrane proteins, directing them to the primary cilium through a Rab8-dependent vesicular transport mechanism.
• BBS7 facilitates BBSome association with RAB3IP/Rabin8, the guanine nucleotide exchange factor for Rab8, thereby promoting Rab8 activation, ciliary membrane extension, and ciliogenesis.
• Subcellularly, BBS7 localizes to the cilium membrane, basal body, centriolar satellites, and centrosome, positioning it at key sites for ciliary trafficking.
• Acetylation of BBS7 has been identified by proteomics, suggesting a potential regulatory post-translational modification.
• Mutations in BBS7 cause Bardet-Biedl syndrome, a ciliopathy marked by obesity, retinal dystrophy, intellectual disability, and other developmental anomalies, underscoring its role in sensory transduction and vision.
• Together with LZTFL1, the BBSome controls ciliary trafficking of Smoothened (SMO), thus linking BBS7 to the sonic hedgehog (SHH) signaling pathway and cilium-dependent developmental processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-270) of human BBS7; consider this when assessing binding epitopes in truncated or mutant constructs.
• For western blot (WB), an expected band near 80 kDa is predicted based on the full-length molecular weight of 80353 Da. Validate band identity using appropriate controls.
• In immunofluorescence/immunocytochemistry (IF/ICC), co-staining with centriolar or ciliary markers may help confirm specific localization to basal bodies and primary cilia. Optimize fixation/permeabilization conditions for these structures.
• This antibody has reported cross-reactivity with mouse and rat BBS7; perform validation in the relevant species model before quantitative assays.
• ELISA applications may require titration under standardized conditions; ensure recombinant BBS7 or lysate coating is validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for diagnostic manufacturers and research laboratories, streamlining the procurement of high-quality IVD raw materials from discovery to commercialization. For studies on primary cilia, BBSome assembly, or ciliopathy mechanisms, this BBS7 polyclonal antibody offers a reliable detection tool. Beyond single antibodies, CamelBio provides validated antibody pairs, bulk recombinant proteins, and sourcing solutions for rare targets—covering your entire workflow with technical and logistical support.