Anti-BAF60a Polyclonal Antibody for WB, IHC-P, ELISA - Q96GM5

Core Product Specifications and Parameters

Biological Background: BAF60a Function and Localization

• BAF60a (also known as SMARCD1 or BRG1-associated factor 60A) is a core subunit of ATP-dependent SWI/SNF chromatin remodeling complexes, which alter DNA-histone contacts to regulate gene transcription. Related references: PMID:8804307, PMID:29374058
• It is a critical component of neural progenitors-specific (npBAF) and neuron-specific (nBAF) chromatin remodeling complexes, playing a key role in the switch from stem/progenitor self-renewal to postmitotic neuronal differentiation.
• BAF60a mediates interactions between nuclear receptors, such as the vitamin D receptor (VDR), and the BRG1/SMARCA4 remodeling ATPase, thereby facilitating hormone-dependent transactivation. Related references: PMID:12917342, PMID:14698202
• The protein is predominantly localized in the nucleus, consistent with its role in chromatin regulation.
• BAF60a is ubiquitously expressed across human tissues including brain, heart, kidney, liver, lung, muscle, pancreas, and placenta.
• Post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination suggest dynamic regulation of its activity.
• Mutations in SMARCD1 are linked to intellectual disability, underscoring its importance in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–120) of mouse BAF60a, which is highly conserved across human, mouse, and rat; this conservation likely contributes to the broad cross-reactivity.
• For Western blot, expect a band at approximately 58 kDa. Consider validating with lysates from brain or other relevant tissues where BAF60a is expressed.
• In immunohistochemistry (paraffin), antigen retrieval conditions may need optimization. Nuclear staining is anticipated; include appropriate controls.
• For ELISA, recombinant protein or peptide coating may be used; ensure proper positive and negative controls to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing platform for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized rare-target procurement. This anti-BAF60a polyclonal antibody supports research into chromatin remodeling and neurodevelopmental disorders, with CamelBio ensuring seamless access to the full spectrum of reagents required from discovery to clinical application.