Anti-ATG2B Polyclonal Antibody for WB, ELISA - Q96BY7

Core Product Specifications and Parameters

Biological Background: ATG2B Function and Localization

• ATG2B, also known as BLTP4B or C14orf103, is an autophagy-related protein that functions as a lipid transfer protein.
• It is required for both autophagosome formation and lipid droplet regulation: it tethers the isolation membrane to the ER and mediates phospholipid transfer for membrane expansion (PubMed:22219374, PubMed:31721365). Related references: PMID:22219374 PMID:31721365
• The protein localizes to preautophagosomal structures, lipid droplets, and the ER membrane.
• Its lipid transfer activity is stimulated by WDR45/WIPI4, which promotes ATG2B association with PI3P-enriched membranes (PubMed:31721365). Related references: PMID:31721365
• ATG2B is a phosphoprotein and belongs to the ATG2 family conserved across eukaryotes.
• The full-length human ATG2B has a predicted molecular weight of approximately 233 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1360-1600 in the C-terminal region of human ATG2B; the antibody may recognize both full-length and truncated forms.
• For Western blotting, expect a band at ~233 kDa; use low-percentage polyacrylamide gels (e.g., 6-8%) and extended transfer times or wet transfer for efficient blotting of high-molecular-weight proteins.
• For ELISA, the antibody can be used as a capture or detection reagent; validate optimal coating concentrations and blocking conditions using recombinant ATG2B antigen or cell lysates.
• Test specificity in your experimental system, especially when analyzing samples from mouse and rat due to potential species-specific epitope differences.
• Polyclonal antibodies may exhibit lot-to-lot variation; include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, offering a one-stop sourcing solution for diagnostic developers and researchers. Our catalog includes validated antibodies, antigen pairs, and ancillary reagents for autophagy and metabolic pathways. For your anti-ATG2B antibody needs and beyond, CamelBio streamlines procurement from concept to clinic, ensuring reliable supply for your assay development pipeline.