Anti-ARFGAP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8N6H7

Core Product Specifications and Parameters

Biological Background: ARFGAP2 Function and Localization

• ARFGAP2, also known as ZNF289, is a zinc finger protein that functions as a GTPase-activating protein (GAP) for ADP-ribosylation factor 1 (ARF1).
• It catalyzes the hydrolysis of ARF1-bound GTP, which may lead to dissociation of coatomer from Golgi-derived membranes, facilitating membrane fusion during ER-Golgi transport.
• Encoded by the ARFGAP2 gene, alternative splicing generates multiple isoforms that may have distinct regulatory roles.
• Localized primarily to the cytoplasm and Golgi apparatus membrane, consistent with its role in vesicular trafficking.
• Contains a zinc finger domain, suggesting metal-binding properties and potential roles in protein-protein or protein-nucleic acid interactions.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its activity or localization.
• Implicated in coatomer-mediated transport, linking it to broader cellular processes of protein secretion and membrane dynamics.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 242-521) spans a region containing functional domains, which may enable detection of multiple ARFGAP2 isoforms by this polyclonal antibody.
• For Western blot, consider validating the antibody using lysates from cell lines with known ARFGAP2 expression (e.g., HeLa, HEK293) and verify the expected molecular weight of approximately 57 kDa.
• In IF/ICC experiments, optimize fixation and permeabilization conditions to preserve the Golgi apparatus localization pattern.
• When using ELISA, determine the optimal dilution empirically for your specific assay format, and include appropriate controls.
• Cross-reactivity with mouse and rat ARFGAP2 is predicted based on sequence homology, but validation in corresponding sample systems is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material sourcing, offering validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents to support research on intracellular trafficking and GTPase regulation. For studies targeting ARFGAP2 and related ER-Golgi transport mechanisms, we provide one-stop access to optimized antibodies and complementary reagents. Our technical services and consulting assist from assay development to clinical application, ensuring streamlined procurement for diagnostic manufacturers and laboratories.