Anti-APOA4 Rabbit Monoclonal Antibody for WB, ELISA - P06727

Core Product Specifications and Parameters

Biological Background: APOA4 Function and Localization

• Apolipoprotein A-IV (APOA4) is a 45372 Da secreted protein primarily synthesized in the intestine and released into plasma.
• It is a major component of high-density lipoproteins (HDL) and chylomicrons, playing a critical role in lipid transport and metabolism.
• APOA4 facilitates the activation of lipoprotein lipase by apolipoprotein C-II and acts as a potent activator of lecithin-cholesterol acyltransferase (LCAT), contributing to reverse cholesterol transport.
• It may be involved in the secretion and catabolism of chylomicrons and very-low-density lipoproteins (VLDL).
• The protein associates with amyloidosis and has been linked to disease variants, highlighting its clinical relevance in lipid disorders.
• Post-translational modifications include phosphorylation, as indicated by the Phosphoprotein keyword.
• APOA4 is extensively studied in 3D-structural analyses, and its reference proteome status underscores its importance in proteomics research.

Experimental Guidance and Technical Tips

• For Western blot, a band around 45 kDa is expected; validate in human plasma or serum samples to confirm endogenous expression.
• For ELISA, the antibody can be used as a capture or detection reagent; consider pairing with another clone specific to a different epitope to build a sandwich assay.
• The immunogen covers the mature protein (aa 21–396), so it likely recognizes post-translationally modified and native forms; verify performance in the relevant sample matrix.
• Cross-reactivity is reported for human; test on other species if needed, though reactivity may be limited.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with comprehensive access to IVD raw materials, including validated monoclonal antibodies like this anti-APOA4 clone. We offer bulk quantities, custom antibody pairs, and ancillary reagents for lipid metabolism research—accelerating your transition from concept to clinic with reliable and scalable supply solutions.