Anti-AKAP5 Rabbit Polyclonal Antibody for WB, ELISA - P24588

Core Product Specifications and Parameters

Biological Background: AKAP5 Function and Localization

• AKAP5, also known as AKAP79 or H21, is a multivalent scaffold protein that anchors cAMP-dependent protein kinase (PKA) to cytoskeletal and organelle-associated structures, thereby targeting cAMP signals to specific intracellular effectors.
  Related references: PMID:1512224
• It interacts with the beta2-adrenergic receptor (beta2-AR), modulating both receptor signaling and PKA-mediated feedback regulation by switching off the beta2-AR cascade.
• AKAP5 contributes to long-term synaptic potentiation by controlling protein trafficking from dendritic recycling endosomes to the plasma membrane, influencing both structural and functional plasticity at excitatory synapses.
  Related references: PMID:25589740
• In hippocampal neurons, AKAP5 recruits the KCNK2/TREK-1 potassium channel to postsynaptic dense bodies and converts it into a leak channel, altering its sensitivity to physiological stimuli.
• It associates with ORAI1 pore-forming subunits of CRAC channels in calcium signaling microdomains, coupling store-operated Ca²⁺ influx to calcineurin and NFAT-dependent transcription.
  Related references: PMID:33941685
• Subcellularly, AKAP5 localizes to the postsynaptic recycling endosome membrane, dendritic projections, and the postsynaptic cell membrane.
• It is predominantly expressed in the cerebral cortex and postsynaptic densities of the forebrain, with lower levels in the adrenal medulla, lung, and anterior pituitary.
• AKAP5 undergoes palmitoylation and calmodulin binding, and its functions are regulated by phosphorylation events.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal region (amino acids 1-270) of human AKAP5; consider using full-length or partial recombinant protein controls to confirm specificity in your assay.
• For Western blotting, AKAP5 is predicted at ~47 kDa; however, post-translational modifications or isoform variants may result in observed bands at different molecular weights. Validate in the relevant sample system.
• The polyclonal nature of this antibody may recognize multiple epitopes, which can enhance sensitivity in ELISA applications; however, lot-to-lot consistency should be verified by the end user.
• Cross-reactivity with mouse AKAP5 has been reported; however, performance in mouse tissues should be experimentally validated, especially given potential species-specific differences in AKAP5 isoforms.
• Avoid freeze-thaw cycles and store according to recommended conditions to maintain antibody integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated supplier of IVD raw materials, offering a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents to support diagnostic manufacturers and research laboratories from concept to clinic. The AKAP5 polyclonal antibody is a valuable tool for studies in neurobiology, signal transduction, and synaptic plasticity. We provide one-stop procurement solutions, including bulk antibody supply, customized assay development components, and rare target sourcing, ensuring you have reliable access to the critical raw materials needed for your projects.