Anti-ACADM Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P11310

Core Product Specifications and Parameters

Biological Background: ACADM Function and Localization

• ACADM (medium-chain specific acyl-CoA dehydrogenase, also known as MCAD, ACAD1, MCADH) catalyzes the stereospecific alpha,beta-dehydrogenation of fatty acyl-CoA thioesters using electron transfer flavoprotein (ETF) as acceptor, preferring saturated C6–C12 chains and longer chains up to C16. Related references: PMID:1970566 PMID:21237683 PMID:2251268
• Electrons from reduced ETF are transferred to the mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase, linking fatty acid oxidation to ATP production. Related references: PMID:15159392 PMID:25416781
• ACADM localizes to the mitochondrial matrix, consistent with its role in the beta-oxidation pathway.
• Exhibits ubiquitous expression, with highest levels detected in heart and skeletal muscle tissues.
• The enzyme is a flavoprotein (FAD-containing) and undergoes regulatory post-translational modifications including acetylation and phosphorylation.
• Mutations in the ACADM gene are linked to medium-chain acyl-CoA dehydrogenase (MCAD) deficiency, an inherited metabolic disorder.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 200–300 of human ACADM; this rabbit monoclonal antibody offers reproducible affinity and specificity.
• In western blot (WB), a major band near 47 kDa is expected; observe for any additional bands that might represent processing intermediates or modified forms.
• For immunohistochemistry (paraffin, IHC-P) and immunofluorescence (IF/ICC), consider mitochondrial-targeted antigen retrieval and permeabilization protocols.
• ELISA applications should be optimized with matched antibody pairs and validated recombinant ACADM protein standards.
• Reported cross-reactivity covers human, mouse, and rat samples; confirm epitope conservation before applying to other species.

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CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-ACADM rabbit mAb. Our supply chain streamlines access to bulk reagents, antibody pairs, and specialized tools for mitochondrial and metabolic disease research, supporting projects from concept to clinic.